Study of Melanin Bleaching After Immunohistochemistry of Melanin-containing Tissues
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چکیده
منابع مشابه
Study of Melanin Bleaching After Immunohistochemistry of Melanin-containing Tissues
Melanin may interfere with immunohistochemical staining. The goal of this study was to investigate the effects of trichloroisocyanuric acid (TCCA) bleaching, potassium permanganate bleaching, and potassium dichromate bleaching on melanin, tissue antigen, and 3,3'-diaminobenzidine (DAB) using melanin-containing and melanin-free tissue samples. Our results demonstrated that all 3 bleaching method...
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Melanin content (percentage by weight) was determined in both pigmented and nonpigmented tissues of Syrian golden hamsters bearing Greene melanoma. Melanin content was also measured in various other melanoma models (B-16 in C57 mice, Harding-Passey in BALB/c mice, and KHDD in C3H mice) and in nine human melanomas, as well as in selected normal tissues. The purpose was to evaluate the possible e...
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Melanin synthesis is associated with virulence for the pathogenic fungus Cryptococcus neoformans. Exposure of nonmelanized C. neoformans 4067 cells to 4 M guanidinium isothiocyanate followed by 6 M HCl at 100 degrees C resulted in complete solubilization of cellular structures. However, exposure of melanized C. neoformans 24067 to the same conditions produced a suspension of black particles. An...
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Bleaching of eumelanin has been studied in model systems consisting of synthetic dopa-melanin and various concentrations of hydrogen peroxide, molecular oxygen, and copper(II) ions at neutral and alkaline pH. The data show that at neutral pH, in the dark, metal-ion-free melanin is very resistant to oxidation by hydrogen peroxide. However, the rate of bleaching of melanin, induced by H2O2 is sig...
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Melanin producing organism was isolated from a soil sample of Ahmednagar district, Maharashtra using Tyrosine casein media. The isolated organism’s morphology and spore arrangement was studied. The isolated organism was suspected to belong to the genus Streptomyces. Parameter optimization of the Tyrosine casein media with respect to carbon and nitrogen source was done. Starch and L-glycine were...
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ژورنال
عنوان ژورنال: Applied Immunohistochemistry & Molecular Morphology
سال: 2015
ISSN: 1541-2016
DOI: 10.1097/pai.0000000000000075